Journal: bioRxiv

Article Title: Cryo-EM structures of α-synuclein filaments from Parkinson’s disease and dementia with Lewy bodies

doi: 10.1101/2022.07.12.499706

Figure Lengend Snippet: Sections from brain regions contralateral to those used for cryo-EM structure determination were stained with monoclonal antibody Syn1 (1:1,000). (a), Cingulate cortex from PD; (b), Cingulate cortex from PDD1; (c), Cingulate cortex from PDD2; (d), Frontal cortex from DLB1; (e), Frontal cortex from DLB2; (f), Cingulate cortex from DLB3. Scale bars: a-c, f, 100 μm; d,e, 50 μm.

Article Snippet: Before blocking, membranes were fixed with 1% paraformaldehyde for 30 min. Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein ( )] (BioLegend) at 1:4,000, Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein ( )] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000.

Techniques: Cryo-EM Sample Prep, Staining

Journal: bioRxiv

Article Title: Cryo-EM structures of α-synuclein filaments from Parkinson’s disease and dementia with Lewy bodies

doi: 10.1101/2022.07.12.499706

Figure Lengend Snippet: PER4 was used at 1:50 in (a-c). (a), PD (Cingulate cortex); (b), PDD1 (Cingulate cortex); (c), DLB3 (Cingulate cortex); Syn303, Syn1 and PER4 were used at 1:4,000 in (d-f). The brain regions used for cryo-EM were also used for immunoblotting. The arrow points to the position of monomeric α-synuclein.

Article Snippet: Before blocking, membranes were fixed with 1% paraformaldehyde for 30 min. Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein ( )] (BioLegend) at 1:4,000, Syn1 [a mouse monoclonal antibody that recognizes residues 91-99 from the NAC region of human α-synuclein ( )] (BD Biosciences) at 1:4,000 and PER4 at 1:4,000.

Techniques: Cryo-EM Sample Prep, Western Blot

Journal: Clinical and Translational Allergy

Article Title: Enhanced expression of endogenous retroviruses and of TRIM28 and SETDB1 in children with food allergy

doi: 10.1002/clt2.12124

Figure Lengend Snippet: Transcription levels of pol genes of HERV‐H, ‐K, ‐W, of Syncytin (SYN)1‐env, SYN2‐env, TRIM28, and SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) in females and males of control children. Median, IQR 25%–75%: HERV‐H pol: females 0.98, 0.72–1.16, males 1.08, 0.84–1.43; HERV‐K pol: females 0.98, 0.72–1.21, males 1.11, 0.83–1.36; HERV‐W pol: females 0.88, 0.72–1.19, males 1.11, 0.91–1.63; SYN1‐env: females 0.98, 0.67–1.54, males 0.97, 0.68–1.31; SYN2‐env: females 0.86, 0.79–1.02, males 0.91, 0.80–1.40; TRIM28: females 1.05, 0.98–1.17, males 0.93, 0.79–1.30; SETDB1: females 1.10, 1.00–1.18, males 0.94, 0.78–1.19. Circles and squares show the median of three individual measurements, horizontal lines the median values. Statistical analysis: Mann–Whitney test was used to compare the transcriptional levels of the target genes between females and males. HERV, human endogenous retrovirus; IQR, interquartile range; RQ, relative quantification

Article Snippet: However, some viral sequences are transcribed and a few encode proteins, such as the Syncytin 1 (SYN1) and Syncytin 2 (SYN2), that play crucial roles in placenta morphogenesis and in feto‐maternal tolerance.

Techniques: Control, MANN-WHITNEY, Quantitative Proteomics

Journal: Clinical and Translational Allergy

Article Title: Enhanced expression of endogenous retroviruses and of TRIM28 and SETDB1 in children with food allergy

doi: 10.1002/clt2.12124

Figure Lengend Snippet: Transcription levels of Syncytin (SYN)1‐env and SYN2‐env in whole blood from children with IgE‐mediated food allergy (IgE‐FA), with food protein‐induced enterocolitis syndrome (FPIES), and from control children (CTRL). Median, IQR 25%–75%: Syn1‐env: Group A 0.80, 0.59–0.99; Group B 0.77, 0.62–1.23; Group C2 0.98, 0.66–1.37; Syn2‐env: Group A 0. 91, 0.60–1.32; Group B 0.94, 0.46–1.23; Group C2 0.90, 0.80–1.17. Circles, squares, and triangles show the median of three individual measurements, horizontal lines the median values. Statistical analysis: One‐Way ANOVA was used to compare the transcriptional levels of SYN‐1 and SYN‐2 between the three groups of children. Mann‐Whitney test was used to compare values of each group of children with each other. ANOVA, analysis of variance; IQR, interquartile range; RQ, relative quantification

Article Snippet: However, some viral sequences are transcribed and a few encode proteins, such as the Syncytin 1 (SYN1) and Syncytin 2 (SYN2), that play crucial roles in placenta morphogenesis and in feto‐maternal tolerance.

Techniques: Control, MANN-WHITNEY, Quantitative Proteomics